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KMID : 0624620100430120830
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BMB Reports
2010 Volume.43 No. 12 p.830 ~ p.835
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Epigenetic modification of retinoic acid-treated human embryonic stem cells
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Cheong Hyun-Sub
Lee Han-Chul
Park Byung-Lae
Kim Hye-Min
Jang Mi-Jin
Han Yong-Mahn
Kim Seun-Young
Kim Yong-Sung
Shin Hyoung-Doo
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Abstract
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Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.
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KEYWORD
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DNA methylation, Epigenetic modification, Gene expression, Human embryonic stem cell, Retinoic acid
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